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We constructed 787 ts strains, covering 497. In this chapter, we describe two shuffling methods for producing ts mutants using a combination of pcr, in vivo recombination, and transformation of diploid strains. In this chapter, we describe two shuffling methods for producing ts mutants using a combination of pcr, in vivo recombination, and transformation of diploid strains heterozygous for a.
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In this paper, we describe an efficient, scalable method to generate ts alleles for each of the essential genes in s. The resultant strains are isogenic to the yko. In this chapter, we describe two shuffling methods for producing ts mutants using a combination of pcr, in vivo recombination, and transformation of diploid strains heterozygous for a.
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In this chapter, we describe two shuffling methods for producing ts mutants using a combination of pcr, in vivo recombination, and transformation of diploid strains.
In this chapter, we describe two shuffling methods for producing ts mutants using a combination of pcr, in vivo recombination, and transformation of diploid strains heterozygous for a. Here, we report detailed protocols for two methodologies that allow the systematic isolation of ts alleles in essential genes of interest. In this chapter, we describe two shuffling methods for producing ts mutants using a combination of pcr, in vivo recombination, and transformation of diploid strains heterozygous for a.
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