Alcian blue and fast red staining of day 21 differentiated chondrocytes polysaccharides / proteoglycans in day 21 differentiated chondrocytes were stained with alcian. Use of alcian blue has historically been a popular staining method in histology especially for light microscopy in paraffin embedded sections and in semithin resin sections. The blue color is due to the presence of copper in the molecule.
Alcian blue stains acid mucosubstances and acetic mucins. This protocol outlines the process for alcian blue staining of 5 dpf embryos fixed with paraformaldehyde (pfa). Principle:alcian blue is a group of polyvalent basic dyes that are water soluble.
It is also used in the. The procedure generally spans 1–2 days,. The 3% acetic acid solution (ph2.5), alcian. Alcian blue is the name given to a family of polyvalent basic dyes.
These dyes can react with compounds containing anionic groups such as acid mucosubstances and acid mucins. Newcomer supply alcian blue stain 1%, ph 2.5 aqueous, an important element in the alcian blue/pas stain procedure is used to differentiate acidic epithelial mucins.